IL-31 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17864873	Invitrogen 500-P249-50UG	50μg
17875583	Invitrogen 500-P249-100UG	100 μg
17885503	Invitrogen 500-P249-1MG	1 mg

Description

AA Sequence of recombinant protein: SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-31. Anti-Human IL-31-specific antibody was purified by affinity chromatography employing an immobilized Human IL-31 matrix. Sandwich ELISA: To detect Human IL-31 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-31 (500-P249Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-31. Western Blot: To detect Human IL-31 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-31 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia, and skin lesions and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.

Specifications

• Antigen: IL-31
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q6EBC2
• Gene ID (Entrez): 386653
• Immunogen: E.coli-derived Recombinant Human IL-31
• Purification Method: Antigen affinity chromatography
• Gene: Il31

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: 1700013B14Rik; IL31; IL-31; ILN; Interleukin; interleukin 31; interleukin-31
• Gene Symbols: Il31
• Primary or Secondary: Primary
• Content And Storage: -20°C