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Invitrogen™ IL-6 Porcine ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ ESIL6
562.40 GBP valid until 2024-12-31
Use promo code "21957" to get your promotional price.
Description
The Porcine Interleukin 6 (IL-6) ELISA quantitates Porc IL-6 in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc IL-6. Principle of the method The Porcine IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.Specifications
P26893 | |
45 pg/mL | |
IL-6 ELISA Kit, 96 Assays, 100μL Sample Volume, 45pg/mL Sensitivity, NP_001239358.1, NP_999564.1 Accession Number, 100628202; 399500 Gene ID, IL6 Gene Symbol, Swine/Porcine Species, Cytokines and Receptors Protein Family, Colorimetric Detection Method | |
Biotin | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
96 Assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
399500 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s) and stop solution | |
96 Tests | |
Immunology | |
2°C to 8°C | |
Porcine | |
4 hr. 45 min. |
40.96-10,000 pg/mL | |
45pg/mL to 10000pg/mL | |
Solid-phase sandwich ELISA | |
Colorimetric | |
Cytokines & Receptors | |
The sandwich ELISA antibody pair detects Porcine IL-6. | |
Swine | |
Colorimetric Microplate Reader | |
IL6 | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
N/A | |
1 hr. 20 min. |