Cytiva Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit

Highly efficient and representative whole-genome amplification with 40 to 60μg yield from nanogram amounts of DNA sample. Cytiva Lifescience™ illustra™ Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products.

Brand:  Cytiva 25-6601-96

Product Code. 12967160

Description

 

• Representative amplification of the whole genome.
• Preformulated, predispensed, single-dose, lyophilized cakes for rapid reaction set-up and greater reproducibility.
• Quick and simple automation-friendly protocol; no thermal cycler required.
• Shipping and storage at room temperature provides easy access and reduced carbon footprint.
• Yields high-quality DNA (e.g. for high-throughput genotyping and DNA archival).
• The reaction mixture is provided as predispensed, single-dose, lyophilized cakes in either strips of 8 tubes, or 96-well plates.
• Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample.
• Amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.
• The average product length is over 10kb.
• Procedure is quick, simple and does not require a thermocycler.
• A typical DNA yield of 12 to 20μg DNA can be achieved in less than two hours from only 10ng of genomic DNA input.
• The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used.
• Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual.
• GenomiPhi amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.

 

Specifications

• For Use With (Application): For whole genome amplification by isothermal strand displacement amplification; typical downstream applications are genotyping (SNP, STR, array CGH) and DNA archiving.
• Includes: Ready-To-Go GenomiPhi V3 cakes, 1 × 96-well plate; 2x Denaturation buffer, 1 × 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps
• No. of Reactions: 96

Safety and Handling

Hazard Statement

• EUH210-Safety data sheet available on request.

Supplemental information

• UNKNOWN ACUTE-72.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
• UNKNOWN AQUATIC-Contains 72.5% of components with unknown hazards to the aquatic environment.