Cytiva IMAC Sepharose™ High Performance Media

For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni²⁺ is not the best choice of metal ion

Brand:  Cytiva 17-0920-07

Product Code. 11534955

Description

• 34μm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
• Charge with transition metal of choice for optimized selectivity
• Small bead size for high performance purification
• High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni²⁺
• Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
• Stable in pH range 3-12 (long term), and in all commonly used buffers

Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.

Specifications

• Flow Rate: <300 cm/hr.
• Particle Size: 34 μm
• Content And Storage: 4°C to 30°C
• For Use With (Application): Purification of Polyhistidine tagged Proteins

• pH Range: 3 to 12
• Product Type: IMAC Sepharose HP
• Quantity: 100 mL

Safety and Handling

Product Identifier

• IMAC sepharose high perfomance

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol