Invitrogen™ ProcartaPlex™ Mouse Immune Response Panel, 64plex

The ProcartaPlex Mouse Immune Response Panel 64plex enables the study of immune function by analyzing 64 protein targets in a single well using Luminex xMAP technology.

Brand:  Invitrogen™ EPX640-20064-901

Product Code. 17942161

Description

The ProcartaPlex Mouse Immune Response Panel 64plex enables the study of immune function by analyzing 64 protein targets in a single well using Luminex xMAP technology.

Analyze 64 cytokine, chemokine, and growth factor targets simultaneously for efficient immune response profiling, biomarker discovery, and validation. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay. This kit is not compatible with the MAGPIX instrument.

Target [bead region]: BAFF (67), BLC (CXCL13) (99), BTC (73), CD27 (84), CTACK (CCL27) (59), ENA-78 (57), Eotaxin (62), Eotaxin-2 (CCL24) (69), G-CSF (12), GM-CSF (42), Granzyme B (49), GRO-a (43), IFN-a (30), IFN-g (38), IL-10 (13), IL-12p70 (39), IL-13 (35), IL-15 (54), IL-16 (68), IL-17A (52), IL-18 (66) ,IL-19 (61), IL-1a (56), IL1-b (19),IL-2 (20), IL-22 (33), IL-23 (37), IL-25 (29), IL-27 (36), IL-28 (64), IL-2Ra (63), IL-3 (14), IL-31 (76), IL-33 (75), IL-33R (78), IL-4 (26), IL-5 (27), IL-6 (28), IL6R/sIL-6R (91), IL-7 (74), IL-7Ra (53), IL-9 (34), IP-10 (22), I-TAC (CXCL11) (81), Leptin (65), LIF (18), MCP-1 (51), MCP-3 (48), MCP-5 (CCL12) (98), M-CSF (21), MDC (CCL22) (88), MIP-1a (47), MIP-1b (72), MIP-2 (55), MIP-3b (CCL19) (87), RANKL (46), RANTES (44), SYB-16 (CXCL16) (58), TARC (CCL17) (79), TECK (CCL25) (89), TNF-a (45), TSLP (70), VEGF-A (25), VEGF-R2 (KDR) (96)

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 μL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

Specifications

• Product Type: Multiplex Panel
• Species: Mouse
• Bead Type: Magnetic
• Format: Multiplex Kit
• Interassay CV: Less than 15%
• Multiplex Size Plex No.: 64
• Sample Type: Serum, Plasma, Cell Culture Supernatants
• Content And Storage: • 4 vials Mouse Standard Mix (lyophilized)
  • 2 vials Control High
  • 2 vials Control Low
  • 1 vial Capture Bead Mix (1X)
  • 1 vial Biotinylated Detection Antibody Mix (1X)
  • 1 bottle Reading Buffer (1X)
  1 bottle Wash Buffer (10X)
  • 1 bottle Streptavidin-PE (1X)
  • 8-Tube Strip
  • Adhesive Film
  • Flat Bottom 96-well Plate, black
  
  Store at 2–8°C.
  
  
• Research Area: Immunology

• Quantity: 96 Tests
• Combinability: Non-Combinable
• Assay Range: See Certificate of Analysis
• For Use With (Equipment): Luminex™ Instruments
• Intraassay CV: Less than 15%
• Product Line: ProcartaPlex™
• Sample Volume: Serum, Plasma: 25 μL; CCS: 50 μL
• Shipping Condition: Wet Ice

For Research Use Only. Not for use in diagnostic procedures.