Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer Set

Designed for use in intracellular staining and flow cytometric analysis and has been specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and increase fluorescence signal-to-noise ratios.

Brand:  Invitrogen™ 88-8824-00

Product Code. 15284728

Includes:

• IC Fixation Buffer
• Permeabilization Buffer (10X)

Description

• In the first step, live cells can be 'fixed' with the Fixation Buffer, which cross- links proteins
• The second step, using the Permeabilization Buffer, creates holes in the membrane and allows the intracellular staining antibodies to enter the cell effectively
• Subsequent washing steps, antibody additions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer
• Final washing step and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer (cat. 00-4222).
• This kit is commonly used to stain cytoplasmic proteins and proteins in the secretory pathway such as chemokines and cytokines
• Not recommended for intranuclear staining. Instead, please refer to the Foxp3/Transcription Factor Buffer Set for nuclear staining
• Before the fixation step, it is recommended to label the live cells using one of the Fixable Viability Dyes and then proceed with fixation

Specifications

• Product Line: eBioscience™
• Product Type: Fixation/Permeabilization Buffer Set
• Quantity: 1 kit
• Solution Type: Fixation/Permeabilization Buffer

Safety and Handling

Product Identifier

• eBioscience Intracellular Fixation & Permeabilization Buffer Set

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 1A
• Serious eye damage/eye irritation Category 1
• Germ cell mutagenicity Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity Category 2

Hazard Statement

• H317-May cause an allergic skin reaction.
• H318-Causes serious eye damage.
• H341-Suspected of causing genetic defects.
• H350-May cause cancer.
• H371-May cause damage to organs.

Precautionary Statement

• P201-Obtain special instructions before use.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P310-Immediately call a POISON CENTER/doctor/.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Paraformaldehyde

For Research Use Only. Not for use in diagnostic procedures.