Invitrogen™ Platinum™ SuperFi™ PCR Master Mix

Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Brand:  Invitrogen™ 12358250

Product Code. 15545043

Description

Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup.

Platinum SuperFi PCR Master Mix retains all the features of Platinum SuperFi DNA Polymerase, a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology. Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Features

• Exceptional >300X Taq fidelity
• High specificity and increased yields with Platinum hot-start technology
• Robust amplification of difficult targets of high GC content
• Room temperature reaction setup and 24-hour bench top stability of pre-assembled reactions
• Amplification of samples with suboptimal purity

Applications

• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Long PCR

Notes

• Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases.
• Platinum SuperFi PCR Master Mix is supplied with separate SuperFi GC Enhancer for difficult and GC-rich templates (˃65% GC).

Specifications

• Content And Storage: 5 boxes of Platinum SuperFi Master Mix, each containing:
  • 2X Platinum SuperFi PCR Master Mix (10 x 1.25 mL)
  • SuperFi GC Enhancer (4 x 1.25 mL)
  • Nuclease-free water (10 x 1.25 mL)
  
  Store at –5°C to –30°C.
• Polymerase: Platinum SuperFi DNA Polymerase
• Quantity: 5 x 500 reactions
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Size (Final Product): 20 kb or less

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Shipping Condition: Wet or Dry Ice
• Fidelity (vs. Taq): 300X
• No. of Reactions: 5 x 500 Reactions
• Reaction Format: SuperMix or Master Mix

For Research Use Only. Not for use in diagnostic procedures.