Invitrogen™ AMV Reverse Transcriptase, Cloned

Can be used to synthesize first-strand cDNA for amplification by PCR or for cloning subsequent to conversion to double-stranded cDNA

Brand:  Invitrogen™ 12328019

Product Code. 10134802

Includes: Cloned AMV RT (50μL at 15U/μL); 1 vial of 5X cDNA synthesis buffer [250mM Tris-acetate pH 8.4, 375mM potassium acetate, 40mM magnesium acetate, and stabilizers]; 1 vial of 100mM DTT; 1 vial of 40mM magnesium acetate; 1 vial of 40mM sodium pyrophosphate

Description

Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV. The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12kb. AMV RT has endoribonuclease H activity.

• Flexibility—works with oligo(dT), random primers, or gene-specific primers
• Thermostability—AMV is active at temperatures up to 55°C
• Multiple applications—first-strand cDNA synthesis for RT-PCR, and sequencing single- and double-stranded DNA and RNA
• Source Purified from insect cells infected with recombinant baculovirus expressing AMV pol
• Concentration: 15U/μL
• Unit definition One unit of cloned AMV RT is the amount of enzyme required to incorporate 1nmole of dexoyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)₂₅ as template-primer
• Optimal reaction temperature: up to 55°C
• Final product size: 12.3kb or less

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: Cloned AMV RT (50μL at 15U/μL); 1 vial of 5X cDNA synthesis buffer [250mM Tris-acetate pH 8.4, 375mM potassium acetate, 40mM magnesium acetate, and stabilizers]; 1 vial of 100mM DTT; 1 vial of 40mM magnesium acetate; 1 vial of 40mM sodium pyrophosphate. Stable for 12 months when stored at -20deg.C. Stability extended by storing at -70deg.C.
• Quantity: 50 reactions (750 units)
• Sensitivity: Low
• Concentration: 15U/μL

For Research Use Only. Not for use in diagnostic procedures.