Invitrogen™ BaculoDirect™ C-Term Expression Kit

Product Code. 10422702

Brand: Invitrogen™ 12562013

Description

Includes

All destination vectors are provided lyophilized and supercoiled.

Requires

Gateway™ entry clone, Gateway™ LR Clonase enzyme mix, and reaction buffer.

Have attR sites for recombination with any attL-flanked fragment, regardless of whether it is entry clone or Ultimate™ RF Clone

Baculovirus Production and Titering, Cloning, Gateway Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: Polyhedrin
• Product Type: Expression Kit
• Content And Storage: The BaculoDirect expression kits include five 0.3 µg vials of BaculoDirect Linear DNA, 125 µL of Cellfectin transfection reagent, frozen Sf9 cells, Gateway LR Clonase enzyme mix, GIBCO Unsupplemented Grace's Insect Medium, and ganciclovir.
  
  Store the BaculoDirect Linear DNA, Cellfectin reagent, and Grace's Medium at +4°C. Store LR Clonase mix at -80°C. Store Sf9 cells in liquid nitrogen. All components are guaranteed stable for 6 months if stored properly.
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Cloning Method: Gateway
• Quantity: 5 transfections
• Vector: BaculoDirect Vectors
• Product Line: BaculoDirect, Gateway

For Research Use Only. Not for use in diagnostic procedures.