Invitrogen™ Biotin-14-dATP

A patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker

Brand:  Invitrogen™ 19524016

Product Code. 10484552

Includes: Biotin-14-dATP is supplied at 0.4mM in 100mM Tris-HCl (pH 7.5), 0.1mM EDTA.

Description

The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP. Biotin-14-dATP can be added to the 3' end of DNA using terminal deoxynucleotidyl transferase. The amount supplied is sufficient for labeling up to 50μg of DNA by nick translation.

Performance and Quality Testing: Purity is evaluated by reverse-phase HPLC analysis.

DNA and RNA Purification and Analysis, DNA Labeling, Nucleic Acid Labeling and Oligo Synthesis

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

• Content And Storage: Biotin-14-dATP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.
• Labeling Method: Direct Labeling
• Product Type: Labeled Nucleotide
• Shipping Condition: Approved for shipment on Wet or Dry Ice

• Format: Liquid
• Label or Dye: Biotin and Other Hapten
• Quantity: 50 nmol
• Concentration: 0.4 mM

For Research Use Only. Not for use in diagnostic procedures.