Invitrogen™ Countess™ Cell Counting Chamber Slides and Holder, disposable

Countess Cell Counting Chamber Slides are conveniently packaged for use with the Countess Automated Cell Counter. Each disposable slide features two enclosed chambers. All Countess Cell Counting Chamber Slides (except Cat. No. C10283) are available with vials of trypan blue.

Includes: Sildes and 20 × 1mL vials of trypan blue (0.4%)

Product Code	Brand	Type	For Use With (Equipment)	Quantity
10399053	Invitrogen™ C10228	Cell Counting Chamber Slide	Countess Automated Cell Counter	50 Slides
12098489	Invitrogen™ C10312	Cell Counting Chamber Slide	Countess Automated Cell Counter	500 Slides
12157220	Invitrogen™ C10313	Cell Counting Chamber Slide	Countess Automated Cell Counter	1250 Slides
12167220	Invitrogen™ C10314	Cell Counting Chamber Slide	Countess Automated Cell Counter	2500 Slides
10665865	Invitrogen™ C10315	Cell Counting Chamber Slide	Countess Automated Cell Counter	5000 Slides
10702404	Invitrogen™ T10282	Trypan Blue Stain	Countess Automated Cell Counter	2 x 1 mL
15594183	Invitrogen™ AMEP4745	Slide Holder	Countess Automated Cell Counter	1 unit, 1 Ea.

Description

Countess Cell Counting Chamber Slides are designed exclusively for automated cell counting on the Countess Automated Cell Counter. These individually packaged slides feature two enclosed chambers and are disposable, offering convenience when performing cell culture. The Countess family of automated cell counters employs the widely used trypan blue staining method for cell counting to identify dead cells and measure cellular viability using brightfield.

The disposable Countess Cell Counting Chamber Slides offer convenience in automated cell counting for processing multiple cell samples, busy cell culture facilities, and easy disposal of contaminated or hazardous samples. They also eliminate the risk of contamination from residual samples. After use, Countess slides can be disposed of as standard biohazard waste. Unlike a hemocytometer, there is no need to clean Countess slides before or after use.

Each Countess Cell Counting Chamber Slide has two separate, enclosed chambers for counting one sample in duplicate or two different samples. Cell counting occurs in a central location of the counting chamber. The volume counted is 0.4 μL, the same as counting four 1-mm² squares in a standard hemocytometer.

The Countess Automated Cell Counter works in three simple steps:

• Mix 10 μL of sample with 10 μL of trypan blue stain or other desired reagent, including ReadyCount™ stains, and pipet into a disposable Countess chamber slide
• Insert the slide into the instrument
• Press 'Count'

That's it—get the total cell count, percent viable cells, and average cell size in as little as 30 seconds.

The Countess Cell Counting Chamber Slides include two vials of trypan blue for brightfield automated cell counting and viability analysis (Cat. Nos. C10228, C10312, C10314, C10313, and C10315). The slides can also be purchased as 1 box of 50 slides without trypan blue (Cat. No. C10283) for use with other stains such as the ReadyCount fluorescent stains. The trypan blue vials are also available for purchase without slides (Cat. No. T10282).

Trypan blue staining is a long-standing and widely used method to measure cellular viability by identifying dead cells. Live cells with intact membranes exclude the dye, while dead cells with compromised membranes become stained, allowing for the differentiation and counting of live and dead cells to assess cellular viability. Trypan blue staining is the method employed by the Countess family of automated cell counters for cell counting, and this 0.4% trypan blue solution is the correct concentration for use on these instruments.

Note: Precipitates are quite common in trypan blue, and typically increase with storage time and freezing. The three approaches below may help minimize trypan blue precipitate in your cell counting samples:

• Sterile filtration of trypan blue using 0.3-micron filter
• Centrifugation or passive settling (avoid mixing or vortexing trypan stock solutions)
• Gentle warming of the 1-mL trypan solution to 37°C for 10 minutes

The Countess Disposable Slide Holder is used to hold the Countess disposable slides. It is designed for use with Countess II FL, Countess 3, and Countess 3 FL automated cell counters and is included with these cell counters. A replacement Countess Disposable Slide Holder (Cat. No. AMEP4745 ) is available for separate purchase. This Countess Disposable Slide Holder is not compatible with the Countess Reusable Slide (Cat. No. A25750).

Safety and Handling

Product Identifier

• X50 CELL COUNTING CHAMBER SLIDE COUNTESS

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 1
• Reproductive toxicity Category 2

Hazard Statement

• H350-May cause cancer.
• H361-Suspected of damaging fertility or the unborn child.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 2,7-Naphthalenedisulfonic acid

For Research Use Only. Not for use in diagnostic procedures.