Invitrogen™ DES™-Inducible Kit with pCoBlast

Small size (3.5kb) to improve DNA yields and increase subcloning efficiency

Brand:  Invitrogen™ K512001

Product Code. 11594946

Includes: Each kit contains 20μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ, Calcium Phosphate Transfection Kit, forward and reverse primers (2μg each), frozen S2 cells, GIBCO Schneider's Drosophila Medium, and the appropriate selection agent.

Description

• Provides expression vector pMT/V5-His and a Blasticidin (pCoBlast) selection
• Uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to culture medium
• C-terminal V5 epitope tag for rapid detection with Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
• To facilitate cloning, set of three vectors-A, B, and C-is provided: Each vector has multiple cloning site in different reading frame relative to coding sequence of C-terminal tag

Inducible Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: MT
• Content And Storage: Each DES™-Inducible Kit contains 20 μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), frozen S2 cells, GIBCO™ Schneider's Drosophila Medium, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Vector: pMT

• Product Type: Inducible Kit
• Protein Tag Position (to your gene): C-terminal
• Cloning Method: Gateway
• Product Line: DES™

For Research Use Only. Not for use in diagnostic procedures.