Invitrogen™ Dynabeads™ CD4 Positive Isolation Kit

The Dynabeads™ CD4 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of high-purity CD4⁺ T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests.

Brand:  Invitrogen™ 11331D

Product Code. 10077282

Includes: 5mL Dynabeads CD4 coated with anti-CD4 monoclonal antibody, and 2mL DETACHaBEAD™ CD4. The kit can process up to 2 x 10⁹ cells.

Description

The Dynabeads™ CD4 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of high-purity CD4⁺ T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells are released from the beads using the DETACHaBEAD™ reagent to obtain bead- and antibody-free cells. Advantages of the Dynabeads™ CD4 Positive Isolation Kit:

• Gentle isolation of CD4⁺ T cells from any sample—no columns required
• Bead- and antibody-free positive isolation of CD4⁺ cells for any downstream assay

About Dynabeads™ CD4 Positive Isolation Kit
The Dynabeads™ CD4 Positive Isolation Kit contains uniform, superparamagnetic beads (4.5 μm diameter) covalently coated with a monoclonal antibody specific for the CD4 membrane antigen predominantly expressed on the helper/ inducer subset of human T cells. The kit also includes a DETACHaBEAD™ reagent that releases the Dynabeads™ from the purified CD4⁺ cells. This gently magnetic isolation process gives highly viable bead- and antibody-free cells to be used in any downstream assay.

Magnetic bead-based separation offers easy handling
Dynabeads™ are mixed with the sample in a tube where they bind to the target cells during a short incubation, after which the bead-bound cells are separated from unbound cells using a magnet. The positively isolated cells are then washed, and DETACHaBEAD™ reagent is added to gently release the cells from the beads. This magnetic bead-based positive isolation method gives both bead- and antibody-free cells. The isolated CD4⁺ cells are phenotypically unaltered and suitable for any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD4⁺ cells. For depletion or isolation of untouched CD4⁺ cells, select from our range of Human CD4⁺ T Cell Isolation Products.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Type: T Cells (CD4)
• Product Type: Positive Cell Isolation Kit
• No. of Cells: Processes ∼2 x 10⁹ cells total
• Product Line: DETACHaBEAD™, DYNAL™, Dynabeads™
• Quantity: 5 mL
• Shipping Condition: Room Temperature
• Target Species: Human

• Content And Storage: Each kit contains 5 mL Dynabeads™ CD4 coated with anti-CD4 monoclonal antibody, and 2 mL DETACHaBEAD™ CD4. The kit can process up to 2 x 10⁹ cells.
  
  Storage Conditions: 2°C to 8°C. Do not freeze.
• Isolation Technology: Positive Isolation
• Output Viability: >95%
• Purity or Quality Grade: Research Grade
• Sample Type: PBMC, Blood
• Starting Material Cell No.: 1 x 10⁷ PBMCs per isolation
• Diameter (Metric): 4.5 μm

For Research Use Only. Not for use in diagnostic procedures.