Invitrogen™ Dynabeads™ SILANE Genomic DNA Kit

For highly predictable and consistent extraction and isolation of genomic DNA from human whole blood, following a simple separation protocol. Isolates linear DNA yields relative to the number of white blood cells (WBC) present in the sample.

Brand:  Invitrogen™ 37012D

Product Code. 12023719

Includes: Dynabeads MyOne; SILANE (5mL, 40mg/mL, see Cat. no. 370-02D for further characteristics), binding/washing/elution buffers and a protocol optimized for isolation of genomic DNA. The kit content is sufficient for 96 isolations.

Description

For highly predictable and consistent extraction and isolation of genomic DNA from human whole blood, following a simple separation protocol. Isolates linear DNA yields relative to the number of white blood cells (WBC) present in the sample. The 1 μm Dynabeads™ have an optimized silica-like surface chemistry and a high specific surface area, providing efficient kinetics and a high capacity.

Their uniform size and surface area ensure highly reproducible results and an improved performance compared to magnetic particles from alternative suppliers. Other particles often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. Dynabeads™ hold reputable Dynal high standards with respect to within- and between-lot reproducibility and automation ability.

Advantages
• Highly predictable binding per mg of beads
• Linear range of DNA yield relative to WBC-count
• High integrity and purity of isolated DNA
• Isolates 10 μg DNA from a 350 μl blood sample using 2 mg (50 μl) Dynabeads™
• Quicker than spin-columns
• More cost-effective, yet performs to the same level as spin-columns
• Automation-friendly (slow sedimentation rate + high magnetic mobility)

Applications
• Isolation of genomic DNA
• From cells and fresh/frozen blood samples
• From blood with a wide range of anticoagulants
• Well suited for automated assays
• Separate kit available for the isolation of viral DNA/RNA (Cat. no. 370-11D)

Additional Info
Validation and customization (bead, buffer, protocol or format) can be made available on a custom OEM basis. For further information, please contact us at: ivd@invitrogen.com

Order Info

Shipping Condition: Room Temperature

Specifications

• Product Type: SILANE Genomic DNA Kit
• Content And Storage: Kit Contains: Dynabeads™ MyOne™ SILANE (5 mL, 40 mg/mL, see Cat. No. 370-02D for further characteristics), binding/washing/elution buffers and a protocol optimized for isolation of genomic DNA.
  
  The kit content is sufficient for 96 isolations. Store at: 2°C to 8°C.
  
• High-throughput Compatibility: High-throughput Compatible
• Particle Size (Metric): 1 μm
• Purification Target: Genomic DNA
• Shipping Condition: Room Temperature

• Sample Type: Blood
• For Use With (Application): Cloning
• Isolation Technology: Magnetic Bead
• Product Line: DYNAL™, Dynabeads™
• Quantity: 96 Preps

Safety and Handling

Product Identifier

• Dynabeads SILANE Genomic DNA Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Guanidine hydrochloride

For Research Use Only. Not for use in diagnostic procedures.