Invitrogen™ Expressway™Lumio™ Expression and Detection System, without vector

For real-time detection of protein synthesis as well as easy in-gel protein detection

Brand:  Invitrogen™ K990060

Product Code. 10216952

Includes: E. coli slyD- Extract, 2.5X IVPS E. coliReaction Buffer, T7 Enzyme Mix, the Feed Buffer, 19 amino acid mix, Methionine, a control plasmid, DNAse/RNAse-free Water, and a Lumio Green Detection Kit for 20 real-time reactions or 100 in-gel applications. In addition to these components, the N-term Expressway Lumio kit also includes 6μg of the pEXP3-DEST vector and the C-term Expressway Lumio kit 6μg of the pEXP4-DEST vector.

Description

• Takes advantage of the Lumio recognition sequence, small, six amino acid sequence (Cys-Cys-Pro-Gly-Cys-Cys)
• Lumio detection reagent binds recognition sequence with high specificity and affinity, resulting in bright fluorescent signal for real-time protein production analysis and immediate in-gel protein detection
• Specialized E. colilysate, derived from slyD mutant, eliminates nonspecific binding of Lumio Green Detection Reagent to endogenous SlyD protein and provides optimal background for detection of recombinant proteins

Cell Free Protein Expression for Functional Analysis, Cell-Free (in vitro) Expression, Enzyme and Protein Activity Assays, Lumio Detection, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays

Specifications

• Product Type: Lumio Expression and Detection System
• Detection Location: In-Gel Detection
• Expression System: Cell-Free
• Quantity: 1 kit

• Content And Storage: The Expressway™ Lumio™ Expression and Detection System includes the E. coli slyD- Extract, 2.5X IVPS E. coli Reaction Buffer, T7 Enzyme Mix, the Feed Buffer, 19 amino acid mix, Methionine, a control plasmid, DNAse/RNAse-free Water, and a Lumio™ Green Detection Kit for 20 real-time reactions or 100 in-gel applications. In addition to these components, the N-term Expressway™ Lumio™ kit also includes 6 μg of the pEXP3-DEST vector and the C-term Expressway™ Lumio™ kit 6 μg of the pEXP4-DEST vector. Store the vectors and the detection reagent at -20°C. Store all other components at -80°C. Guaranteed stable for 6 months when properly stored.
• Expressed Protein Type: Water Soluble Protein
• Expression Mechanism: Cell-Free Expression
• Product Line: Expressway™, Lumio™

For Research Use Only. Not for use in diagnostic procedures.