Invitrogen™ Expressway™ Maxi Cell-Free E. coli Expression System

Uses efficient, coupled transcription/translation reaction to produce milligram quantities of soluble, functionally active protein in 4 to 6 hours

Brand:  Invitrogen™ K990097

Product Code. 10553083

Includes: Designed to perform 200 x 25μL,5 x 1mL, or 1 x 5mL (initial volume) reactions or one to several large reactions. Includes the E.coli slyD- Extract, 2.5X IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, T7 Enzyme Mix, and a positive expression control vector. The Expressway Maxi Cell-Free E. coli Expression System with pEXP5-NT/TOPO and pEXP5-CT/TOPO also includes the pEXP5-NT/TOPO and pEXP5-CT/TOPO TA Expression Kits. Each kit contains the pEXP5-NT/TOPO or pEXP5-CT/TOPO vector, other reagents to facilitate TOPO Cloning, and TOP10 Chemically Competent E. coli.

Description

• Procedure can be performed in single reaction tube and is easily scalable without need for specialized equipment
• TOPO TA Cloning expression vectors (5-min cloning with 95% efficiency) are provided for optimal expression results
• In addition to all advantages of open expression system, produces high levels of recombinant protein that may be easily detected and purified for various downstream applications
• Proprietary formulation of Feed Buffer boosts reaction productivity so that more than one milligram of protein is produced in 2mL reaction within 4 to 6 hours
• The formulation of lysate enables protein synthesis using either circular or linear templates
• One kit is supports 200 x 50 reactions, which can be accommodated by 2 x 96-well plates
• Without feed buffer, such reactions need only 2 hours
• Once positive expression is detected, scale-up protein production can be performed with large cell-free format or in E. coli cell-based systems

Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: T7
• Content And Storage: The Expressway™ Maxi Cell-Free Expression System is designed to perform two hundred 25-μl, five 1-ml, or one 5-ml (initial volume) reactions or one to several large reactions, depending on your needs. This system includes the E.coli slyD- Extract, 2.5X IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, T7 Enzyme Mix, and a positive expression control vector. The Expressway™ Maxi Cell-Free E. coli Expression System with pEXP5-NT/TOPO™ and pEXP5-CT/TOPO™ also includes the pEXP5-NT/TOPO™ and pEXP5-CT/TOPO™ TA Expression Kits. Each kit contains the pEXP5-NT/TOPO™ or pEXP5-CT/TOPO™ vector, other reagents to facilitate TOPO™ Cloning, and One Shot™ TOP10 Chemically Competent E. coli. Store the vectors at -20°C. Store all other components at -80°C. Guaranteed stable for 6 months when properly stored.
• Expression System: Cell-Free
• Protein Tag: N-Terminal
• Quantity: 1 kit
• Product Line: Expressway™

• Product Type: Cell-Free Expression System
• Expressed Protein Type: Water Soluble Protein
• Expression Mechanism: Cell-Free Expression
• Cloning Method: TOPO-TA
• Vector: pEXP5-NT/TOPO, pEXP5-CT/TOPO

For Research Use Only. Not for use in diagnostic procedures.