Invitrogen™ Flp-In™-293 Cell Line

Cells contain a single stably integrated FRT site at a transcriptionally active genomic locus

Brand:  Invitrogen™ R75007

Product Code. 10164282

Includes: 1 x 10⁷ cells are supplied frozen in 1mL of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen.

Description

Flp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted integration of a Flp-In expression vector ensures high-level expression of your gene of interest. Flp-In-293 cell line was created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™- resistant clones. Flp-In-293 cells work well with Flp-In vectors that express a gene from the CMV promoter (e.g., pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO™, and pSecTag/FRT/V5-His-TOPO

Constitutive Expression, Mammalian Expression, Mammalian Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration

Specifications

• Content And Storage: 1 x 10E7 cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.
• Species: Human
• Product Line: Flp-In™

• Cell Line: Flp-In-293
• No. of Cells: 1 mL
• Quantity: 1 mL

For Research Use Only. Not for use in diagnostic procedures.