Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R E. coli

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

Brand:  Invitrogen™ K252020

Product Code. 10286662

Includes: TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, Mach1-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.

Description

• Fast, 5-minute TOPO TA Cloning delivers 95% recombinants
• 3'-T overhangs simplify cloning of Taq-amplified PCR products
• Fast growing MachI cells shorten cloning time
• Sequencing primer sites located close to cloning insertion site so sequences more insert, less vector first time
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

Specifications

• Cloning Method: TOPO™ with Gateway™
• No. of Reactions: 20 reactions
• Product Type: TA Cloning Kit

• Content And Storage: The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored
• Product Line: One Shot™
• Vector: pCR

For Research Use Only. Not for use in diagnostic procedures.