Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R E. coli and PureLink™ Kit

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

Brand:  Invitrogen™ K252002

Product Code. 10369892

Includes: pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers; TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes.

Description

• pCR™8 TOPO TA vector contains minimal MCS, so sequence more of insert and less of vector
• Patented att sites make pCR™8 vector is simplest way to create an entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, saving valuable research time
• pCR™8/GW/TOPO TA Cloning Kit features fast growing E. coli that shortens cloning time
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

Specifications

• Cloning Method: TOPO™ with Gateway™
• No. of Reactions: 20 preps
• Product Type: TA Cloning Kit

• Content And Storage: pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers; One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink™ Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes. Store all components of the PureLink™ kit at room temperature. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• Product Line: One Shot™
• Vector: pCR

For Research Use Only. Not for use in diagnostic procedures.