Invitrogen™ Gateway™ pDONR™221 Vector

Enables rapid cloning of one or more genes into virtually any protein expression system

Brand:  Invitrogen™ 12536017

Product Code. 11568706

Includes: 6μg pDONR221 vector, lyophilized in TE buffer, pH 8.0.

Description

• Designed to create entry clone following PCR with attB-modified custom primers
• With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
• PCR product is directionally cloned with efficiencies typically >99%
• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™
• In addition, pDONR221 and pDONR™/Zeo vectors have pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use

Cloning, Gateway Cloning

Specifications

• Product Type: Gateway System Destination Expression Vector
• Cloning Method: Gateway
• Vector: pDONR

• Content And Storage: 6 μg pDONR221™ vector, lyophilized in TE buffer, pH 8.0. Upon receipt, store at -20°C.
• Quantity: 6 μg
• Product Line: Gateway™, pDONR™

For Research Use Only. Not for use in diagnostic procedures.