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Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli
Use highly efficient, 5-minute cloning strategy to directionally clone a blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System
Brand: Invitrogen™ K242020
447.48 GBP valid until 2024-09-27
Use promo code "21650" to get your promotional price.
Code : 50
Additional Details : Weight : 0.00450kg
Includes: pENTR/SD/D-TOPO vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, OneShot TOP10 Chemically E. coli, S.O.C. Medium, and a pUC19 control plasmid.
Description
- Blunt-end PCR products clone directionally into pENTR/SD/D-TOPO entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes
- Rapidly shuttle cloned genes between multiple vector systems
- Reliable performance
- Directional cloning vector for entry to Gateway System and optimized for prokaryotic expression
- For access to Gateway System, PCR amplify the gene of interest and add product straight to the provided topoisomerase charged pENTR/SD/D-TOPO vector, incubate 5 minutes, and transform the provided competent E. coli cells
- Resulting attL containing Gateway Entry clones are ready for efficient recombination with choice of Gateway Destination vectors
- pENTR/SD/D-TOPO vectorincludes T7 gene 10 translational enhancer and ribosome binding site (RBS) for optimal expression of native protein after recombination with prokaryotic Gateway destination vectors
- Can also use vector for expression in other hosts systems by recombination with different destination vectors
- Vector has M13 and T7 primer sequencing sites and attL recombination sites flanking PCR product insertion site, allowing clones to be easily sequence-verified and recombined into choice of attR containing Gateway destination vectors
- Kanamycin resistance gene and pUC origin are used for selection and high-copy propagation in E. coli
- Four base overhang on vector pairs with four base sequence designed into forward primer used in PCR reaction to provide directionality to the topoisomerase ligation reaction
Cloning, Gateway Cloning
Specifications
Directional TOPO™ | |
20 reactions | |
TOPO Cloning Kit |
pENTR™⁄SD⁄D-TOPO™ Cloning Kit contains pENTR⁄SD⁄D-TOPO™ vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, OneShot™ TOP10 Chemically E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store Competent E. coli at -80°C. Store all other components at -20°C. | |
One Shot™ | |
pENTR |
For Research Use Only. Not for use in diagnostic procedures.