Invitrogen™ MultiSite Gateway™ Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway™ destination vector

Product Code. 10312203

Brand: Invitrogen™ 12537100

Description

Includes

Include vectors and primers, Mach1 T1^R Chemically Competent Cells, and LR Clonase II Plus and BP Clonase II enzymes.

For flexible cloning of up to four DNA fragments into Gateway destination vector

• Using specifically designed att sites for recombinational cloning, clone two, three, or four DNA fragments into any Gateway Destination vector containing attR1 and attR2 sites
• Resulting expression clone is ready for downstream expression and analysis applications
• Clone multiple DNA fragments into one vector without using restriction enzymes or ligases
• Full advantage of wide selection of Gateway Destination vectors or create vectors
• Replace multiple plasmid transfections with single vector carrying all DNA elements of interest
• MultiSite Gateway Pro Kits are available for cloning two, three, or four DNA fragments and for flexible and customized configuration of DNA fragments into a Gateway Destination vector

Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Multi-Gene Expression, Multisite Gateway, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: None (Promoterless)
• Product Type: Gateway System Destination Expression Vector
• Content And Storage: The MultiSite Gateway™ Pro Kits include vectors and primers as indicated in Table 1, One Shot™ Mach1™ T1^R Chemically Competent Cells, and LR Clonase™ II Plus and BP Clonase™ II enzymes. Store cells at -80°C. Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• Cloning Method: Gateway
• Quantity: 20 reactions
• Vector: MultiSite Gateway Vectors
• Product Line: Gateway
• For Use With (Application): Clone your own Promoter, Multi-Gene Expression

Safety and Handling

Product Identifier

• MultiSite Gateway Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway destination vector

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : Proteinase, tritirachium album serine

For Research Use Only. Not for use in diagnostic procedures.