Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

Brand:  Invitrogen™ K490001

Product Code. 10605583

Includes: Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA

Description

• Proofreading enzyme is used: fewer errors in cloned genes
• Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
• CMV promoter: high expression levels
• C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
• 6xHis tag for rapid protein purification on nickel-chelating resin

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Dry Ice

Specifications

• Constitutive or Inducible System: Constitutive
• Promoter: CMV
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Quantity: 20 Reactions
• Product Line: TOPO™, pcDNA™

• Delivery Type: Transfection
• Product Type: TOPO Expression Kit
• Content And Storage: Box 1 (Store at -20°C):
  • 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)
  • 10 μl dNTP Mix
  • 50 μl Salt Solution
  • 1 ml Sterile Water
  • 20 μl T7 Sequencing Primer (0.1 μg/μl)
  • 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)
  • 10 μl Control PCR Template (0.1 μg/μl)
  • 10 μl Control PCR Primers (0.1 μg/μl each)
  • 10 μl Expression Plasmid (0.5 μg/μl)
  
  Box 2 (Store at -80°C):
  • 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)
  • 21 × 50 μl TOP10 Cells
  • 50 μl pUC19 Control DNA
• Cloning Method: Directional TOPO
• Vector: Directional TOPO Vectors, pcDNA
• For Use With (Application): Constitutive Expression

For Research Use Only. Not for use in diagnostic procedures.