Invitrogen™ pIB/V5-His TOPO™ TA Expression Kit

Offers five-minute cloning of Taq-amplified PCR products directly into the pIB/V5-His-TOPO expression vector

Brand:  Invitrogen™ K89020

Product Code. 10174742

Includes: Each pIB/V5-His TOPO TA Expression Kit contains two boxes. The pIB/V5-His TOPO box contains 200ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO vector, dNTPs, 10X PCR buffer, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, salt solution, and pIB/V5-His/CAT. The box contains high-efficiency, single-use, 50μL aliquots of chemically competent TOP10 E. coli cells, S.O.C. medium, and pUC19 supercoiled plasmid control.

Description

• The OpIE2 promoter for constitutive expression
• Includes Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification using nickel-chelating resin

Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: OplE2
• Content And Storage: Each pIB/V5-His TOPO™ TA Expression Kit contains two boxes. The pIB/V5-His TOPO™ box contains 200 ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO™ vector, dNTPs, 10X PCR buffer, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, salt solution, and pIB/V5-His/CAT. Store at -20°C. The One Shot™ box contains high-efficiency, single-use, 50 μl aliquots of chemically competent TOP10 E. coli cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. All reagents are guaranteed stable for 6 months when properly stored.
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Quantity: 20 reactions
• Product Line: InsectSelect™, TOPO™

• Product Type: TOPO TA Expression Kit
• Protein Tag Position (to your gene): C-terminal
• Cloning Method: TOPO-TA
• Vector: pIB, TOPO-TA Cloning Vectors

For Research Use Only. Not for use in diagnostic procedures.