Invitrogen™ Platinum™ Blue PCR SuperMix 96, Skirted

Ideal for automatic hot-start amplification of DNA fragments with improved specificity over Taq DNA polymerase

Brand:  Invitrogen 12580031

Product Code. 11538716

Includes: Platinum Taq is supplied with 10X PCR buffer (200mM Tris-HCl (pH 8.4), 500mM KCl), and 50mM MgCl₂. Platinum Taq PCR_x DNA Polymerase is supplied with 10X PCR buffer, 50mM MgCl₂ , 50mM MgSO₄ , 10X PCR_x Enhancer Solution, and 10X PCR_x Amplification Buffer. SuperMixes are supplied in a 1.1X concentration and contain Platinum Taq, dNTPs, MgSO₄ ,and buffer. Platinum Blue PCR SuperMix also contains loading/tracking dye.

Description

Derived from recombinant Taq DNA polymerase by binding of a thermolabile inhibitor containing monoclonal antibodies to Taq DNA polymerase. During initial denaturation step of PCR, the inhibitor is denatured and active Taq DNA polymerase is released into the reaction. Platinum Blue PCR SuperMix is a PCR master mix containing Platinum Taq DNA Polymerase, dNTPs, premixed loading/tracking dye, buffer, magnesium and salts. The blue tracking buffer will not inhibit your PCR performance. After PCR cycling, simply load your PCR product directly onto an agarose gel. Platinum TaqPCR_x DNA Polymerase is Platinum Taq DNA Polymerase supplied with the PCR_x Enhancer System for optimizing PCR of problematic and/or GC-rich templates. 10X PCR_x Enhancer Solution with 10X PCR_x Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Taq DNA polymerase.

• Improved specificity and yields of PCR compared to Taq DNA polymerase without “hot-start”
• PCR products up to 5kb in size
• Room temperature reaction assembly

Applications: Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR.
Unit Definition: One unit of Platinum TaqDNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonu-cleotide into DNA in 30 min. at 74°C.

Hot Start PCR, PCR, PCR and Real-Time PCR, Routine PCR

Order Info

Shipping condition: Wet Ice

Specifications

• Content And Storage: Platinum Taq is supplied with 10X PCR buffer (200mM Tris-HCl (pH 8.4), 500mM KCl), and 50mM MgCl₂ . Platinum TaqPCR_x DNA Polymerase is supplied with 10X PCR buffer, 50mM MgCl₂ , 50mM MgSO₄ , 10X PCR_x Enhancer Solution, and 10X PCR_x Amplification Buffer. SuperMixes are supplied in a 1.1X concentration and contain Platinum Taq, dNTPs, MgSO₄ ,and buffer. Platinum Blue PCR SuperMix also contains loading/tracking dye.
• For Use With (Application): Real Time PCR (qPCR)
• Size (Final Product): 5kb or less

• Quantity: 5 plates
• Overhang: 3'-A

For Research Use Only. Not for use in diagnostic procedures.