Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Includes: Platinum PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50μL each are provided (4 x 1.125mL). Platinum PCR SuperMix High Fidelity Components: 22U/mL complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum Taq Antibody; 66mM Tris-SO₄ (pH 8.9); 19.8mM (NH₄)₂SO₄; 2.4mM MgSO₄; 220μM dNTPs; and stabilizers.

Product Code	Brand	No. of Reactions
10545013	Invitrogen™ 12532016	100 Reactions
10175622	Invitrogen™ 12532024	5000 Reactions

Description

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.

High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic hot-start PCR.

Benefits of Platinum PCR SuperMix, High Fidelity

• Six-times higher fidelity than Taq DNA polymerase
• Amplifies fragments up to 15 kb
• Minimizes PCR optimization
• Room-temperature reaction assembly

Applications

• High-fidelity PCR
• Cloning
• Mutagenesis

Notes

• Platinum PCR SuperMix, High Fidelity, produces products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs.
• Hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Fidelity (vs. Taq): 6X
• Overhang: Mixed
• Size (Final Product): 15 kb or less

• Polymerase: Platinum Taq DNA Polymerase High Fidelity
• Shipping Condition: Wet or Dry Ice
• Concentration: 1.1X
• Hot Start: Built-In Hot Start
• Reaction Format: SuperMix or Master Mix

For Research Use Only. Not for use in diagnostic procedures.