Invitrogen™ IL-8 Human ProQuantum Immunoassay Kit

Measure cytokine proteins with small sample consumption and high sensitvity on a qPCR instrument

Brand:  Invitrogen™ A355755

Product Code. 17133099

Description

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8,secreted by endothelial cells and monocytes,which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8,first isolated from osteosarcoma cells,contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1),granulocyte chemotactic protein 1 (GCP-1),monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

Specifications

• Accession Number: P10145
• Assay Sensitivity: <0.01pg/mL
• Product Type: ProQuantum
• Target Species: Human
• For Use With (Equipment): qPCR Instruments
• Gene Symbol: GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1
• Label or Dye: FAM™
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Test Time: 30 min.

• Assay Range: 0.0128 to 5000 pg/mL
• Detection Method: Taqman™ Real-time PCR
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Cytokine protein quantitation
• Gene ID (Entrez): 3576
• Kit Contents: Assay Dilution Buffer<Br>Antibody-Conjugate A<Br>Antibody-Conjugate B<Br>Antibody-Conjugate Dilution Buffer<Br>Standard<Br>Master Mix<Br>Ligase
• Quantity: 5 x 96 Tests
• Sample Volume: Serum, 2-5 μL; Plasma, 2-5 μL; Supernatant, 2-5 μL
• Target: Human
• Total Assay Time: 2 hr.