Invitrogen™ ProQuest™ Two-Hybrid System with Gateway™ Technology

An in vivo yeast-based system for identifying interactions between two proteins

Brand:  Invitrogen™ PQ1000101

Product Code. 10043562

Includes: The ProQuest Two-Hybrid System includes yeast expression vectors pDEST32, pDEST22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay.

Description

• Reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media
• Allow rapid and easy generation of bait and prey constructs, and facilitate downstream application
• Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors (ARS/CEN) to control over-expression and increase reproducibility
• Three different reporter genes (HIS3, URA3, and lacZ) with independent promoter regions to rapidly weed out false positives
• URA3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid
• A set of strong-to-weak Gateway-based control interactions to evaluate results and assess the strength of the interaction being tested

Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis, Yeast Two-Hybrid

Specifications

• Product Type: Two-Hybrid System
• Content And Storage: The ProQuest™ Two-Hybrid System includes yeast expression vectors pDEST™32, pDEST™22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase™ II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay. Store kit components at -80°C. Guaranteed stable for six months when properly stored.
• Expression System: Yeast

• Reporter Gene: Beta-Gal (lacZ), HIS3, URA3
• Assay: Two-Hybrid Assay
• Product Line: Gateway™, ProQuest™

For Research Use Only. Not for use in diagnostic procedures.