Invitrogen™ PureLink™ Genomic DNA Mini Kit

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.

Brand:  Invitrogen™ K182002

Product Code. 10458843

Description

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:

• Kit flexibility—one kit works with a variety of sample types and sizes
• Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
• Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use. Columns are newly configured to bind and release DNA more efficiently. Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A₂₆₀/A₂₈₀ measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells. The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀, indicating no detectable contamination from carryover of protein or guanidine.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: • 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature
  • 45 mL PureLink Genomic Digestion Buffer; room temperature
  • 50 mL PureLinkGenomic Wash Buffer 1; room temperature
  • 37.5 mL PureLink Genomic Wash Buffer 2; room temperature
  • 50 mL PureLink Genomic Elution Buffer; room temperature
  • 5 mL RNase A (20 mg/mL); room temperature
  • 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage
  • 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature
  • 5 x 100 PureLink Collection Tubes; room temperature
  
  
• Scale: Mini
• Elution Volume: 25 to 200 μL
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• Quantity: 250 Preps
• Test Time: Up to 15 min. after cell lysis
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Cells: ≤10⁶
  Tissue: ≤25 mg
  Mouse Tail: 1 cm
  Rat Tail: 0.5 cm
  Whole Blood: ≤1 mL
  Yeast: ≤10⁷ cells
  FFPE: ≤8 sections of 5-15 μM thickness
  Oragene™ Preserved Saliva: ≤4 mL
  Blood Spots: ≤5 punches

• Isolation Technology: Silica Spin Column
• Sample Type: Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
• Final Product Type: gDNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Shipping Condition: Room Temperature
• Yield: ≤40 μg

Safety and Handling

Product Identifier

• PURELINK GENOMIC DNA KIT (250)

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Guanidine hydrochloride, Proteinase, tritirachium album serine

For Research Use Only. Not for use in diagnostic procedures.