Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit

The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.

Product Code	Brand	Includes	Quantity
10257072	Invitrogen™ K210006		10 Preps
12809702	Invitrogen™ K210006		10 Preps
10464402	Invitrogen™ K210007		25 Preps

Description

The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 μg plasmid DNA from 100–200 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

• Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
• High-purity, low-endotoxin product- pure enough for mammalian cell transfection
• Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification

• PureLink HiPure Plasmid DNA Purification Kits use patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
• Resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal
• Plasmid preparations can typically be completed in less than 2 hours

Contaminant-free DNA

• Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
• Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A₂₆₀/A₂₈₀ ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
• Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
• Purification time is two hours

Order Info

Shipping Condition: Room Temperature

Specifications

• Format: Column
• Scale: Maxi
• Elution Volume: 15 mL
• For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
• Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
• Product Line: PureLink™
• Purity: Transfection Grade
• Target: BAC DNA, Plasmid DNA
• Test Time: 2 hr.

• Isolation Technology: Anion Exchange Resin
• Sample Type: Bacterial Culture
• Final Product Type: BAC DNA, Plasmid DNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
• Product Type: Plasmid MaxiPrep Kit
• Shipping Condition: Room Temperature
• System Type: PureLink™
• Yield: 1000 μg

Safety and Handling

Product Identifier

• PureLink HiPure Plasmid Maxiprep Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information

• MIXTURE LIST-Contains : acetic acid

For Research Use Only. Not for use in diagnostic procedures.