Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.

Brand:  Invitrogen™ K210004

Product Code. 10083762

Description

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350 μg plasmid DNA from 15–25 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

• Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
• High-purity, low-endotoxin product- pure enough for mammalian cell transfection
• Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification

• PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients
• The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal
• Plasmid preparations can typically be completed in less than 2 hours

Contaminant-free DNA

• Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
• Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A₂₆₀/A₂₈₀ ratio of greater than 1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
• Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
• Purification time is two hours

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: • 100 mL Resuspension Buffer (R3)
  • 550 μL RNase A
  • 100 mL Lysis Buffer (L7)
  • 100 mL Precipitation Buffer (N3)
  • 250 mL Equilibration Buffer (EQ1)
  • 500 mL Wash Buffer (W8)
  • 125 mL Elution Buffer (E4)
  • 15 mL TE Buffer
  • 25 HiPure Columns
  • 5 Column Holders
  
  Store all components at room temperature.
• Isolation Technology: Anion Exchange Resin
• Elution Volume: 5 mL
• For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
• No. of Reactions: 25 Preps
• Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
• Product Type: Plasmid MidiPrep Kit
• Purity: Transfection Grade
• Target: BAC DNA, Plasmid DNA
• Test Time: 2 hr.

• Format: Column
• Sample Type: Bacterial Culture
• Final Product Type: BAC DNA, Plasmid DNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
• Product Line: PureLink™
• Quantity: 25 Preps
• Shipping Condition: Room Temperature
• System Type: PureLink™
• Yield: 350 μg

Safety and Handling

Product Identifier

• PureLink HiPure Plasmid Midiprep Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information

• MIXTURE LIST-Contains : acetic acid, Sodiumlauryl sulfate, Sodium hydroxide

For Research Use Only. Not for use in diagnostic procedures.