Invitrogen™ PureLink™ Quick Gel Extraction Kit

The PureLink™ Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages.

Brand:  Invitrogen™ K210012

Product Code. 10749243

Includes: Gel Solubilization Buffer (L3)
Wash Buffer (W1)
Elution Buffer (E5); (10 mM Tris-HCl, pH 8.5)
Quick Gel Extraction Columns
Wash Tubes
Recovery Tubes

Description

The PureLink™ Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in ∼30 minutes using PureLink™ silica membrane-based quick gel extraction columns. For your convenience, purification protocols are provided for centrifugation and for vacuum.

Advantages of using PureLink™ Quick Gel Extraction Kit:
• Purify DNA fragments from TAE and TBE agarose gels of various percentages and melting points
• Complete the procedure in ∼30 minutes
• Easily purify DNA fragments from 40 bp to 10 kb from gels
• Obtain high recovery of DNA fragments
• Bind and purify up to 15 μg DNA with one column
• Purify DNA fragments that are high quality and show reliable performance in PCR, restriction enzyme digestion, cloning, and labeling

Note: The PureLink™ Quick Gel Extraction Kit is not designed to purify supercoiled plasmid DNA or genomic DNA from agarose gels. Only linear DNA fragments may be purified from gels using this kit.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: • 2 x 90 mL Gel Solubilization Buffer L3; room temperature
  • 16 mL Wash Buffer W1; room temperature
  • 15 mL Elution Buffer E5; room temperature
  • 50 Quick Gel Extraction Columns with Collection Tubes; room temperature
  • 50 Recovery Tubes; room temperature
  
  
• Sample Type: Agarose Gels, Gel Samples
• Final Product Type: DNA
• Green Features: Less hazardous, sustainable packaging
• Label or Dye: Ethidium Bromide, SYBR Safe
• Shipping Condition: Room Temperature
• Yield: 15 μg (Binding capacity)

• Isolation Technology: Silica Spin Column
• Elution Volume: 50 μL
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Test Time: 30 min.
• Starting Material Amount: ≤400 mg

Safety and Handling

Product Identifier

• 0.12 LT PURELINK GEL EXTRACTION KIT

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Skin corrosion/irritation Category 1 C

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Thiocyanic acid, compound with guanidine

For Research Use Only. Not for use in diagnostic procedures.