Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit

The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns.

Brand:  Invitrogen™ K210011

Product Code. 10694823

Includes: Resuspension Buffer (R3)
RNase A (20 mg/mL)
Lysis Buffer (L7)
Precipitation Buffer (N4)
Wash Buffer (W9)
Wash Buffer (W10)
TE Buffer (TE)
Spin Columns
Wash and Recovery Tubes

Description

The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns. Features of the PureLink™ Quick Plasmid Miniprep Kit:

• Fast—ready-to-use plasmid DNA typically in about 30 min
• Flexible—formats work with vacuum and centrifugation platforms

Reliable and cost-effective isolation of plasmid DNA
The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30–45 min, using the PureLink™ spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 μg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.

Compatible with flexible formats
The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A₂₆₀/A₂₈₀ of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a variety of downstream applications
Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.

Order Info

Shipping Condition: Room Temperature

Specifications

• Concentration: 20 mg/mL
• Content And Storage: • Resuspension buffer
  • RNase A (20 mg/mL)
  • Lysis buffer
  • Precipitation buffer
  • Wash buffers
  • TE buffer
  • Spin columns in wash tubes
  • Elution tubes
  
  
• Isolation Technology: Silica Spin Column
• Sample Type: Bacterial Culture
• For Use With (Application): Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation
• No. of Reactions: 250 Preps
• Product Line: PureLink™
• Quantity: 250 Preps
• Target: Plasmid DNA

• Column Type: Spin Column
• Format: Spin Column
• Scale: Mini
• Final Product Type: Plasmid DNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
• Product Type: Quick Plasmid MiniPrep Kit
• Shipping Condition: Room Temperature
• Test Time: 30 to 45 min.

Safety and Handling

Product Identifier

• PureLink Quick Plasmid Miniprep Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information

• MIXTURE LIST-Contains : Acetic acid

For Research Use Only. Not for use in diagnostic procedures.