Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit

The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants.

Brand:  Invitrogen™ 12280050

Product Code. 10013922

Includes:

• 32mL Viral Lysis buffer (L22)
• 15mL 5X Wash Buffer (W5)
• 2 x 1.6mL Proteinase K (20mg/mL in storage buffer)
• 310μg lyophilized Carrier RNA
• 15.5mL Sterile, RNAase-free water
• 50 Viral Spin Columns with Collection Tubes
• 2 x 50 Wash Tubes (2.0mL)
• 50 Recovery Tubes (1.5mL)

Description

The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases and is suitable for use in downstream applications that allow viral detection and genotyping.

The PureLink Viral Mini Kit has been used to purify viral dsDNA from orthopoxviruses including cowpox virus, variola virus, monkeypox virus, vaccinia virus, and HERV.

The PureLink Viral RNA/DNA Mini Kit provides the following advantages:
• Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 μL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 μL to allow sensitive downstream analysis

Order Info

Shipping Condition: Room Temperature

Specifications

• Column Type: Spin Column
• Isolation Technology: Silica Spin Column
• Scale: Mini
• Final Product Type: Viral RNA and DNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Shipping Condition: Room Temperature
• Yield: 5 μg (Binding capacity)

• Content And Storage: • 310 μg Carrier RNA; –20°C
  • 32 mL Viral Lysis Buffer L22; room temperature
  • 15 mL Wash Buffer WII; room temperature
  • 2 x 1.6 mL Proteinase K; room temperature, or 4°C for long-term storage
  • 15.5 mL RNase-free Water E3; room temperature
  • 50 Viral Spin Columns with Collection Tubes; room temperature
  • 2 x 50 Wash Tubes; room temperature
  • 50 Recovery Tubes; room temperature
  
  
• Sample Type: Cells, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM)
• Elution Volume: 10 to 50 μL
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
• Quantity: 50 Preps
• Test Time: 45 min.
• Starting Material Amount: ≤500 μL

Safety and Handling

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : thiocyanic acid, proteinase

For Research Use Only. Not for use in diagnostic procedures.