Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector, without competent cells

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Product Code	Brand	Bacterial or Yeast Strain	No. of Reactions	Vector
10266302	Invitrogen™ K207040	Not Included	40 Reactions	pCRII
10442832	Invitrogen™ K207020	Not Included	20 Reactions	pCRII

Description

The T7 and Sp6 promoters of the pCRII vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCRII cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

• Fast and convenient: 15-minute, room-temperature ligation
• Efficient: blue/white screening and >80% clones with correct insert
• Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free: eliminates any enzymatic modifications of PCR product
• Streamlined: does not require use of PCR primers that contain restriction sites
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and Sp6 promoters for in vitro RNA transcription and sequencing
• Versatile polylinker with flanking EcoR I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing
• Cloning Method: Restriction Enzyme/MCS

• How TA Cloning Works
• Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
• Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
• Allows PCR inserts to ligate efficiently with vector
• Kit Configurations
• TA Cloning Kit is offered in a variety of configurations: with One Shot INVF' Chemically Competent E. coli (K2050-01 and K2050-40), One Shot TOP10F' Chemically Competent E. coli (K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20- and 40-reaction kit sizes

Cloning, PCR Cloning

Specifications

• Bacterial or Yeast Strain: Not Included
• Content And Storage: This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.
  
  Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• For Use With (Application): PCR Cloning
• Product Type: Cloning Kit
• Vector: pCRII

• Cloning Method: TA Cloning
• Format: Kit
• Product Line: TA Cloning™
• Promoter: T7, SP6

For Research Use Only. Not for use in diagnostic procedures.