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Invitrogen™ Terminal Deoxynucleotidyl Transferase, recombinant
Performance and quality tested
£125.00 - £317.00
Specifications
Content And Storage | Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C. |
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Enzyme | Terminal Deoxynucleotidyl Transferase |
Compatible Buffer | 5X Buffer, Reaction Buffer |
Product Type | TdT |
Alert:
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical. See MSDS.
Includes: Vial of 5X buffer [500mM potassium cacodylate (pH 7.2), 10mM CoCl2 , 1mM DTT]
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
10573093
|
Invitrogen™
10533065 |
500 U |
£125.00
500 units |
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11578626
|
Invitrogen™
10533073 |
3 x 500 U |
£317.00
Pack of 3 |
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Description
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is DNA polymerase that catalyzes addition of deoxynucleotides to 3fi hydroxyl terminus of DNA.
- Source: Purified from baculovirus clone of calf thymus TdT
- Performance and Quality Testing: Endonuclease, 3' and 5' exodeoxyribonuclease, and levels of incorporation tested
- Unit Definition: One unit incorporates 1nmol dATP into acid-precipitable material in 1 hr. at 37°C, using d(pA)50 as primer
- Unit Reaction Conditions: 0.2M potassium cacodylate (pH 7.2), 10mM MgO4 C4 H6 , 1mM 2-mercaptoethanol, 0.5mg/mL BSA, 100 flM d(pA)50, 1mM [3H]dATP, and enzyme in 0.15mL for 1 h at 37°C
Cloning; Restriction Enzyme Cloning; Homopolymer tailing of vector and insert for cloning; Labeling oligonucleotides with biotin, 32P- or 35S-label; Apoptosis (TUNEL)
Order Info
Shipping Conditions: Approved for shipment on wet or dry ice
Specifications
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C. | |
Terminal Deoxynucleotidyl Transferase | |
5X Buffer, Reaction Buffer | |
TdT |
For Research Use Only. Not for use in diagnostic procedures.