Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP 10 Chemically Competent E.coli, no manual

For fast, efficient cloning and subsequent in vitro transcription/translation, use Invitrogen's TOPO TA Cloning Dual Promoter Kit with pCR™II vector

Brand:  Invitrogen™ KNM460040

Product Code. 10422882

Description

By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol and yielding up to 95% recombinants.

• 3′-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient and validated restriction sites including EcoR I flanking your insert for subsequent excision or sub-cloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
• Includes OneShot™ TOP10 F′ Competent cells for added convenience and highest cloning efficiency

Cloning, PCR Cloning

Specifications

• Product Type: Kit, Cloning
• Bacterial or Yeast Strain: TOP10
• Quantity: 50 Reactions

• Includes: Box 1: Topoisomerase I-activated pCRII-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
• For Use With (Application): Cloning, PCR Cloning