Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

Offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

Brand:  Invitrogen™ K280002

Product Code. 10379892

Includes: Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Description

Kit includes linearized and topoisomerase I-activated pCR™-Blunt II-TOPO™ Vector for 5-minute benchtop ligations without ligase.

• ccdB gene for positive selection
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and Zeocin resistance genes for your choice of selection in E. coli
• SP6 promoter/primer site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening
• Selected Zero Blunt TOPO PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis

Cloning, PCR Cloning

Order Info

Shipping Condition: Dry Ice

Specifications

• Bacterial or Yeast Strain: TOP10
• Cloning Method: Blunt TOPO™
• Format: Kit
• Product Type: PCR Cloning Kit
• Quantity: 25 Reactions

• Cell Type: Chemically Competent
• Content And Storage: Box 1:
  • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
  • Salt solution
  • dNTPs
  • Control template
  • M13 forward and reverse primers
  • Sterile water
  
  Store at -5 to -30°C.
  All reagents are stable for 6 months when properly stored.
  
  Box 2:
  • One Shot™ Chemically Competent or Electrocomp™ E. coli
  • S.O.C. medium
  • Supercoiled pUC19 control plasmid
  
  Store at -68 to -85°C.
• Product Line: One Shot™
• Promoter: SP6
• Vector: Blunt DNA Cloning Vectors

For Research Use Only. Not for use in diagnostic procedures.