Invitrogen™ KDalert™ GAPDH Assay Kit with Manual

KDalert™ GAPDH Assay Kit with Manual

Brand:  Invitrogen™

Product Code. 10065704

Description

The Ambion™ KDalert™ GAPDH Assay Kit is for the reliable measure of GAPDH enzyme activity in cultured human, mouse, or rat cells in less than 30 minutes using a microplate fluorometer. The kit includes sufficient reagents for 375 reactions.

Assess GAPDH siRNA delivery in 1/3 the time for 1/3 the cost of real-time PCR

Analyze 1–96 samples simultaneously

Measure both GAPDH siRNA-induced knockdown AND transfection-induced toxicity

Compatible with a wide variety of cells and a broad range of culture conditions

The KDalert GAPDH Assay Kit is an ideal positive control for transfection optimization experiments and also measures transfection induced cytoxicity. It is designed for use with Ambion™ Silencer™ GAPDH siRNA.

Rapid, Time-Saving Procedure
Use the assay to optimize siRNA transfection by transfecting individual cell samples with a GAPDH siRNA and a negative control siRNA. Two to three days after transfection, simply add the included cell lysis buffer to the cells, incubate for 20 minutes, add the diluted master mix of assay reagents, and read the increase in fluorescence four minutes later using a microplate or standard fluorometer. The assay procedure can be completed in about 30 minutes with minimal sample handling.

One Assay for Two Readouts
Because GAPDH is expressed at relatively constant levels, the assay can also be used to monitor transfection agent induced toxicity. For this analysis, GAPDH enzyme activity from negative control siRNA-transfected cells is compared to that of untreated cells. Reduced GAPDH activity in negative control-transfected cells compared to non-transfected cells is an indication that the transfection-induced cytotoxicity.

Accessory Products:
The KDalert™ Kit is designed for use with Silencer™ GAPDH siRNAs (SKUs #AM4605, AM4633, AM4634, AM4624, AM4632, or AM4631). Additional KDalert™ Lysis Buffer (SKU #AM8790G) is also available separately.

Specifications

• Description: KDalert™ GAPDH Assay Kit with Manual, 375 rxns, GAPDH Assays, Fluorescent Detection Method, Chemical substrate, GAPDH (Enzyme) Target, Absorbance, Fluorescence Intensity Technique, Used For RNAi Applications
• Detection Method: Fluorescence
• Substrate Type: Chemical
• For Use With (Application): RNAi, epigenetics and non-coding RNA research, RNAi, siRNA
• Quantity: 375 Reactions
• Target: GAPDH (enzyme)

• Assay: GAPDH
• Product Type: KDalert™ GAPDH Assay Kit
• Sufficient For: 375 rxn(s)
• Kit Contents: KDalert lysis buffer can be stored at –20°C or 4°C, solution A, solution B, and solution C should be stored at –20°C, GAPDH enzyme should be stored at or below –70°C
• Regulatory Status: For research use only (RUO)

For Research Use Only. Not for use in diagnostic procedures.