Thermo Scientific™ Kinase Tracer 178, 25 μM

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody.

Brand:  Thermo Scientific™ PV5593

Product Code. 10340074

Description

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.

Specifications

• For Use With (Equipment): Microplate Reader
• Target: Kinases (Multiple)
• Assay: Kinase Binding Assay
• Assay Entry: Biochemical binding/kinetic
• Product Type: Kinase Tracer
• Format: 384-well plate
• Shipping Condition: Dry Ice

• Product Line: LanthaScreen™
• For Use With (Application): TR-FRET
• Content And Storage: Store at -20°C.
• Label or Dye: Alexa Fluor 647
• Quantity: 25 μL
• Readout: Kinetic
• Target Entry: PTK6, DDR2, BMPR1A, RAF1 (cRAF) Y340D Y341D, ACVR1B, WNK2, TEC, DDR1, EPHB4, ERBB4, KIT V654A, TGFBR1, NLK, TGFBR2, EPHB3, BRAF V599E, BRAF, ACVR2B, ACVR2A

For Research Use Only. Not for use in diagnostic procedures.