Thermo Scientific™ Klenow Fragment (10 U/μL)

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Product Code	Brand	Concentration	Quantity
10130800	Thermo Scientific™ EP0051	10 U/μL	300 U
10795071	Thermo Scientific™ EP0052	10 U/μL	1500 U

Description

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.

Highlights

• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
• Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications

• DNA blunting by fill-in 3'-overhangs
• Random-primed DNA labeling
• Labeling by fill-in 5'-overhangs of dsDNA
• DNA sequencing by the Sanger method
• Site-specific mutagenesis of DNA with synthetic oligonucleotides
• Second strand synthesis of cDNA

Specifications

• Concentration: 10 U/μL
• Polymerase: DNA Polymerase I
• Product Type: Klenow Fragment

For Research Use Only. Not for use in diagnostic procedures.