L-Leucine-4-nitroanilide, 99%, Thermo Scientific Chemicals

Description

It is used to detect/evaluate functionality of amino peptidases. The compound is used in an ELISA assay to detect biological functionality of chicken amino peptidase produced by E. coli. It is also employed to characterize the native IRAP (placental leucine amino peptidase). Leu-pNA, Chromogenic Substrate is documented for use with pure samples as well as cell extracts in vitro.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
It is used to detect/evaluate functionality of amino peptidases. The compound is used in an ELISA assay to detect biological functionality of chicken amino peptidase produced by E. coli. It is also employed to characterize the native IRAP (placental leucine amino peptidase). Leu-pNA, Chromogenic Substrate is documented for use with pure samples as well as cell extracts in vitro.

Solubility
Soluble in DMSO (>100 mM), and methanol (>25 mM).

Notes
Store away from oxidizing agents. Keep the container tightly closed in a well ventilated place.

Specifications

Chemical Identifiers

• CAS: 4178-93-2
• Molecular Formula: C12H17N3O3
• Molecular Weight (g/mol): 251.29
• MDL Number: MFCD00063681
• InChI Key: AXZJHDNQDSVIDR-NSHDSACASA-N
• Synonym: l-leucine-4-nitroanilide, h-leu-pna, l-leucine-p-nitroanilide, s-2-amino-4-methyl-n-4-nitrophenyl pentanamide, 2s-2-amino-4-methyl-n-4-nitrophenyl pentanamide, pentanamide, 2-amino-4-methyl-n-4-nitrophenyl-, s, l-leucyl-p-nitroanilide, pentanamide, 2-amino-4-methyl-n-4-nitrophenyl-, 2s, h-leu-pna.hcl, l-leu p-nitroanilide
• PubChem CID: 2733285
• IUPAC Name: (2S)-2-amino-4-methyl-N-(4-nitrophenyl)pentanamide
• SMILES: CC(C)C[C@H](N)C(=O)NC1=CC=C(C=C1)[N+]([O-])=O

• Melting Point: 89°C to 90°C
• Beilstein: 2218433
• Solubility Information: Soluble in DMSO (>100 mM),and methanol (>25 mM).
• Quantity: 1 g
• Formula Weight: 251.29
• Percent Purity: 99%
• Chemical Name or Material: L-Leucine-4-nitroanilide

Safety and Handling

Product Identifier

• L-Leucine-4-nitroanilide

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin sensitiser Category 1

Hazard Statement

• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : L-Leucine-4-nitroanilide

RUO – Research Use Only