Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (4X)

Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.

Brand:  Thermo Scientific Chemicals J60015.AD

Product Code. 15402869

Description

Laemmli SDS sample buffer is used for protein sample preparation, which is used in the Laemmli SDS-PAGE system. It is a widely used system for protein electrophoresis. Further, it is employed in the polyacrylamide gel analysis of proteins. In addition to this, it is utilized to reduce disulfide bonds in order to denature proteins.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Laemmli SDS sample buffer is composed of several compounds, including 375 mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 6% β-mercaptoethanol, 0.03% bromophenol blue
• The SDS compound present in the Laemmli SDS sample buffer binds noncovalently to proteins. SDS is negatively charged and can mask the intrinsic charge of the protein it binds

Application

• Laemmli SDS sample buffer is used to denature proteins and for protein sample preparation to be used in the SDS-PAGE system
• This sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of the running gel
• It is also used for polyacrylamide protein gel analysis

Specifications

• Color: Blue
• Product Type: Protein Sample Loading Buffer
• For Use With (Application): Laemmli SDS-PAGE

• Concentration: 4X
• Form: Liquid
• Quantity: 50 mL

Safety and Handling

Product Identifier

• Laemmli SDS sample buffer, reducing (4X)

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 2
• Serious eye damage/eye irritation Category 1
• Skin sensitiser Category 1

Hazard Statement

• H302-Harmful if swallowed.
• H311-Toxic in contact with skin.
• H317-May cause an allergic skin reaction.
• H318-Causes serious eye damage.
• H332-Harmful if inhaled.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.

Supplemental information

• MIXTURE LIST-Contains : sodium lauryl sulphate, 2-mercaptoethanol, 1,3-propanediol 2-amino-2-(hydroxymethyl)-hydrochloride,

RUO – Research Use Only