Invitrogen™ LanthaScreen™ TR-FRET BACE1 Assay Kit

LanthaScreen™ TR-FRET BACE1 Assay Kit

Brand:  Invitrogen™ PV4748

Product Code. 10283888

Description

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides found in extracellular amyloid plaques of Alzheimer’s disease (AD). In some cases, early-onset familial AD can be attributed to a Swedish mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that focus on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase.

Invitrogen’s LanthaScreen™ TR-FRET BACE1 assay provides sensitive high-throughput screening for potential inhibitors of beta-secretase. The kit uses a terbium (Tb)-labeled anti-biotin antibody and a fluorescein-labeled BACE1-biotin substrate in a homogeneous TR-FRET assay format (Figure 1).



Contents and Storage:

The LanthaScreen™ TR-FRET BACE1 Assay Kit contains BACE1 protein, fluorescently labeled BACE1 substrate, Tb-labeled anti-biotin antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

• Description: LanthaScreen™ TR-FRET BACE1 Assay Kit, 800 x 20μL, Liquid, β-Secretase Assay, Used in Protein Assays and Analysis, Peptide-Based Substrate Properties, Beta Secretase (BACE1) Target Enzyme, TR-FRET Technique, Beta Secretase Substrate
• Product Type: LanthaScreen™ TR-FRET BACE1 Assay Kit
• Sufficient For: 800 x 20μL
• Quantity: 800 x 20 μL
• Storage Requirements: -5°C to -30°C

• Assay: β-Secretase
• Substrate Type: Beta Secretase
• For Use With (Application): Protein assays and analysis
• Regulatory Status: For research use only. Not for use in diagnostic procedures

For Research Use Only. Not for use in diagnostic procedures.