missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, goat
LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit (Goat)
Brand: Invitrogen™ PV4839
This item is not returnable.
View return policy
Description
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15142:The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay:
When running the LanthaScreen™ TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound’s ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, addition-only format.

Specifications
Tb (Terbium) | |
Microplate Reader | |
384-well Plate | |
Biochemical competitive binding | |
End Point | |
NR1I2, PXR(SXR) | |
Competitive Binding Assay, TR-FRET | |
TR-FRET PXR (SXR) Competitive Binding Assay Kit |
Fluorescence | |
800 x 20 μL assays | |
Store in ultra-cold freezer (-68 to -85°C). | |
8856 | |
Dry Ice | |
LanthaScreen™ | |
PXR | |
800 x 20 μL assays |
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
5 results found
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Invitrogen™ LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, goat > 800 x 20μL assays
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction