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Thermo Scientific™ LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit
The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR).
Brand: Thermo Scientific™ A15142
Description
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4839:
The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay:
When running the LanthaScreen™ TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound's ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, 'addition-only' format.
Specifications
Green | |
LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, Rabbit, 800 x 20μL Assays, Green, 384-Well Plate Format, Fluorescent Detection Method, Nuclear Receptors Druggable Target, Tb (Terbium) Label, TR-FRET Readout, Nuclear Receptor Assay | |
Microplate Reader | |
Competitive ligand binding | |
For research use only. Not for use in diagnostic procedures | |
TR-FRET PXR(SXR) assay buffer | |
Biochemical Assay | |
TR-FRET (Binding) | |
Nuclear Receptor Assay, TR-FRET | |
TR-FRET PXR (SXR) Competitive Binding Assay Kit |
Tb (Terbium) | |
Fluorescence | |
Tb-anti-GST Antibody (Rabbit), PXR(SXR)-LBD, GST, fluormone PXR(SXR) green, TR-FRET PXR(SXR) assay buffer, 1M DTT | |
800 x 20 μL Assays | |
384-well Plate | |
• Tb-anti-GST Antibody (Rabbit) (store at -20°C) • PXR(SXR)-LBD, GST (-80°C) • Fluormone PXR(SXR) Green (-20°C) • TR-FRET PXR(SXR) Assay Buffer (4°C) • 1M DTT (-20°C or -80°C) |
|
Nuclear Receptors | |
LanthaScreen™ | |
PXR | |
800 x 20 μL |
For Research Use Only. Not for use in diagnostic procedures.