Invitrogen™ Lipocalin-2 (LCN2) Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMLCN2X5

Product Code. 17135759

Description

The Mouse Lipocalin-2 (LCN2) ELISA quantitates Ms Lipocalin-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Lipocalin-2. Principle of the method The Mouse Lipocalin-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.

Specifications

• Accession Number: P11672
• Assay Sensitivity: 0.02 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 16819
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plates, Wash Buffer, SAV-HRP, Biotinylated Detection Antibody, Chromogen, Adhesive Plate Covers, Assay Diluent Concentrate, Standard, Stop Solution
• Quantity: 5 x 96 Tests
• Research Discipline: Inflammation
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.024 ng/mL to 100 ng/mL
• Calibration Range: 0.02 ng/mL to 100 ng/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, O x 40, Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: 24p3,AW212229,NRL,Sip24
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 1 μ;L; Serum, 1 μ;L; Supernatant, 100 μ;L
• Synonym: RP23-161B9.11-003, 24p3, AW212229, Sip24
• Test Time: 1 hr. 20 min.