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Ly-49I Monoclonal Antibody (YLI-90), FITC, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 11-5895-85
Description
Description: The YLI-90 monoclonal antibody reacts with the B6 mouse Ly-49I receptor. Ly49I, a member of the Ly49 family with inhibitory function, is a homodimer type II transmembrane protein and contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM). YLI-90 binds to subpopulations of NK, NKT and T cell lineages in the C57Bl/6 strain. This antibody does not bind to cells from BALB/c, SJL or AKR/J mice. Applications Reported: YLI-90 has been reported for use in flow cytometric analysis. Applications Tested: The YLI-90 antibody has been tested by flow cytometric analysis of C57Bl/6 and BALB/c mouse splenocytes and can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.
Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.Specifications
Ly-49I | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
0 | |
Klra9 | |
Affinity chromatography | |
RUO | |
16640 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
YLI-90 | |
FITC | |
Klra9 | |
5E6; Killer cell lectin-like receptor 3; killer cell lectin-like receptor subfamily A, member 9; Klra3; Klra9; Ly-49; Ly49C; Ly-49c; Ly49I; Ly49I receptor; Ly-49I receptor; LY49I1; Lymphocyte antigen 49c; Nk2.1; T-cell surface glycoprotein Ly-49C | |
Mouse | |
500 μg | |
Primary | |
Mouse | |
Antibody | |
IgG1 κ |
For Research Use Only.