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Lysis buffer BQ1, Macherey Nagel Bioanalysis™
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Quantity:
125 mL
Description
Flexible handling of multiples of 8 samples is achieved by use of a reusable column holder. Parallel lysis of large sample numbers can be performed in standard microcentrifuge tubes or in 96 well blocks alternatively.NucleoSpin™ Blood QuickPure binding strips are to be used in a centrifuge with deep buckets allowing processing of NucleoSpin™ 8 well strips stacked on 96 well blocks. For further information contact our Technical Support Team.The method is based on a specially treated silica membrane which speeds up the procedure by a combined washing/drying step. The number of washing and drying steps is reduced from 3 to only 1, therefore hands-on time is minimised.Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of proteinase K. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin™ Blood QuickPure binding strips are created by addition of ethanol to the lysate. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer and can be used directly for PCR*, Southern blotting or any kind of enzymatic reaction. The kit allows isolation of 4μg to 6μg of pure genomic DNA from 200μL whole blood with an A260/280 ratio between 1.60 and 1.90 and a typical concentration of 40ng/μL to 60ng/μL.Kit components: NucleoSpin™ Blood QuickPure binding strips, buffers, proteinase K, accessories.
† For preparation of 300μL samples increased volumes of lysis buffer BQ1 required.
- Silica membrane technology
- Manual processing
- Yield: 4μg to 6μg
- Sample material: human or animal whole blood (200μL/300μL†)
- Elution volume: 7.5μL to 100μL
- Binding capacity: 60μg
- Preparation time: 60min/12 strips
† For preparation of 300μL samples increased volumes of lysis buffer BQ1 required.
Specifications
Specifications
Quantity | 125 mL |
Safety and Handling
Signal Word
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- MIXTURE LIST-Contains : guanidine hydrochloride
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