Applied Biosystems™ MagMAX™ Wastewater Ultra Nucleic Acid Isolation Kit

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from wastewater samples.

Brand:  Applied Biosystems™ A52606

Product Code. 17321909

Description

• The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from wastewater samples
• The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications
• Wastewater and sewage surveillance is a useful tool for monitoring the regional spread of viral and bacterial pathogens
• Surveillance at different levels of operatio has been shown to be a reliable way to detect the SARS-CoV-2 virus before it becomes widespread in a population
• Wastewater surveillance can be used to monitor other viral targets such as influenza and norovirus
• However, wastewater and sewage samples are some of the most challenging when it comes to extraction of RNA and DNA

Features of the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit include:

• Automation-ready protocols designed for KingFisher Flex, KingFisher APEX, and KingFisher Duo systems
• Fast procedure allows for 24–96 samples to be processed in ∼45 minutes
• Compatible with wastewater and sewage concentrated via ultracentrifugation, precipitation, filtration, or bead-based viral enrichment and non-concentrated samples
• Includes methods for 200 μL to 500 mL starting volumes of wastewater
• Elution volumes ranging from 50 to 100 μL

Specifications

• Content And Storage: • 80 mL Lysis Buffer; room temperature
  • 50 mL Binding Solution; room temperature
  • 200 mL Wash Buffer; room temperature
  • 25 mL Elution Solution; room temperature
  • 4 mL Proteinase K; room temperature
  • 2 mL DNA/RNA Binding Beads; room temperature
  
  
• Sample Type: Sludge, Wastewater
• Final Product Type: RNA and DNA
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
• Quantity: 100 preps
• Test Time: Up to 29 min. when using automated protocol

• Isolation Technology: Magnetic Bead
• Elution Volume: 50 to 200 μL
• For Use With (Equipment): KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
• High-throughput Compatibility: High-throughput Compatible
• Shipping Condition: Room Temperature
• Starting Material Amount: Sludge: ≤200 mg
  Wastewater: ≤500 mL

Safety and Handling

Product Identifier

• MagMAX Wastewater Ultra Nucleic Acid Isolation Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 1 C
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P363-Wash contaminated clothing before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Thiocyanic acid, Proteinase

For research use only. Not for use in diagnostic procedures.